Antifreeze protein NMR sensor to detect water molecular reorientation in the surface of ice
نویسندگان
چکیده
منابع مشابه
NMR Characterizations of the Ice Binding Surface of an Antifreeze Protein
Antifreeze protein (AFP) has a unique function of reducing solution freezing temperature to protect organisms from ice damage. However, its functional mechanism is not well understood. An intriguing question concerning AFP function is how the high selectivity for ice ligand is achieved in the presence of free water of much higher concentration which likely imposes a large kinetic barrier for pr...
متن کاملHigh water mobility on the ice-binding surface of a hyperactive antifreeze protein.
Antifreeze proteins (AFPs) prevent uncontrolled ice formation in organisms exposed to subzero temperatures by binding irreversibly to specific planes of nascent ice crystals. To understand the thermodynamic driving forces and kinetic mechanism of AFP activity, it is necessary to characterize the hydration behavior of these proteins in solution. With this aim, we have studied the hyperactive ins...
متن کاملMimicry of ice structure by surface hydroxyls and water of a beta-helix antifreeze protein.
Insect antifreeze proteins (AFP) are much more effective than fish AFPs at depressing solution freezing points by ice-growth inhibition. AFP from the beetle Tenebrio molitor is a small protein (8.4 kDa) composed of tandem 12-residue repeats (TCTxSxxCxxAx). Here we report its 1.4-A resolution crystal structure, showing that this repetitive sequence translates into an exceptionally regular beta-h...
متن کاملIce surface reconstruction as antifreeze protein-induced morphological modification mechanism.
The crystal growth process by which fish antifreeze proteins (AFPs) and antifreeze glycoproteins (AFGPs) modify the ice morphology is analyzed in the AFP-ice system. A newly identified AFP-induced surface reconstruction mechanism enables one-dimensional helical and irregular globular ice binding surfaces to stabilize secondary, kinetically less stable ice surfaces with variable face indices. No...
متن کاملUse of NMR to detect water within nonpolar protein cavities.
response to antibody to CD3. These results 176, 269 (1992). Katona, J. Immunol. 138, 2826 (1987). 9H. Arase, N. Arase, K. Nakagawa, R. A. Good, K. 18. F. D. Finkelman et al., ibid. 151, 1235 (1993). thus leave open the question of whether Onoe, Fur. J. Immunol. 23, 307 (1993). 19. CD8-HSA'0 thymocytes were prepared by one-step early cytokine production by NK1 .1 + T 10. 0. Lantz and A. Bendelac...
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ژورنال
عنوان ژورنال: The Journal of Chemical Physics
سال: 2009
ISSN: 0021-9606
DOI: 10.1063/1.3223181